Fig. 3.
Effect of Y-27632 on low O2 tension-induced ATP release from erythrocytes [red blood cells (RBC)]. Healthy human erythrocytes were equilibrated with a gas mixture containing 15% O2-6% CO2-balance nitrogen (Po2 = 107 ± 1 mmHg) in the absence (filled bars) or presence (open bars) of Y-27632. After a 30-min incubation period, baseline erythrocyte ATP release was established (normoxia). The gas mixture was changed to 0% O2-6% CO2-balance nitrogen (Po2 = 11 ± 1 mmHg), and ATP release from erythrocytes was measured (low O2). During the course of the experiment, temperature (37°C), pH (7.37 ± 0.02), and Pco2 (36 ± 0.4) were held constant. Values are reported as means ± SE. *Different from baseline (P < 0.05). †Different from all other values (P < 0.01), n = 13.