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. 2011 Sep 2;301(5):H1891–H1896. doi: 10.1152/ajpheart.00603.2011

Fig. 6.

Fig. 6.

Effect of diamide in the absence and presence of Y-27632 on low O2 tension-induced ATP release from erythrocytes. Healthy human erythrocytes were equilibrated under normoxic conditions in the presence of vehicle (saline), diamide, and diamide plus Y-27632. Baseline ATP release was not different among the vehicle-treated, diamide-treated, and diamide- plus Y-27632-treated erythrocytes (30.2 ± 3.6, 36.6 ± 4.40, and 33.0 ± 4.5 nmol/4 × 108 erythrocytes, respectively). Exposure to low O2 tension increased ATP release from vehicle-treated and diamide- plus Y-27632-treated erythrocytes (47.5 ± 3.7 and 50.7 ± 11.2 nmol/4 × 108 erythrocytes, respectively), but not from diamide-treated erythrocytes (46.4 ± 2.50 nmol/4 × 108 erythrocytes). During the course of the experiment, temperature (37°C), pH (7.32 ± 0.01), and Pco2 (41 ± 0.6 mmHg) were held constant. Values are reported as changes from baseline ± SE. *Different from baseline values (P < 0.05), n = 7.