Fig. 3.

Acrolein increases myocardial nitrative stress. A: WB and quantitation for phospho (P)-endothelial nitric oxide synthase (eNOS)-Ser¹¹⁷⁷ and total eNOS performed on total cardiac homogenates from mice 24 h after a single oral dose of acrolein (5 mg/kg) or vehicle (n = 5/group). Hela, Hela cell lysate. B: WB and densitometry for eNOS dimer and monomer performed on cardiac homogenates derived from mice chronically fed acrolein or water for 48 days (n = 4–5/group). C: immunofluorescent stains for protein-nitrotyrosine (NT, red) with 4′,6-diamidino-2-phenylindole (DAPI) costain for nuclei (blue) in hearts harvested from acrolein-fed and control-fed mice as in B, along with fluorescence quantitation (control, n = 3; acrolein, n = 5). Peroxynitrite-treated sections were used as a positive control. *P < 0.05 vs. control.