Fig. 5.

Confocal microscopic images of terminal deoxytransferase-mediated dUTP nick-end labeling (TUNEL) staining in hearts from mice chronically fed acrolein or vehicle (control) and quantitation of TUNEL-positive nuclei. Myocytes were stained with anti-troponin I (red), and nuclei were stained with DAPI (blue). TUNEL-positive nuclei appear green-cyan on the overlaid images from the acrolein-exposed heart as shown in the magnified (zoom) image. Arrows denote a TUNEL-positive nucleus. Scale bar: standard magnification 20 μm; zoomed magnification 5 μm. n = 5/group.