Fig. 3.

The [NO] along the lymphatic bulb region is highly influenced by the proximity of valvular tissue. Four locations from the origin of the bulb, the origin of the valve leaflets on the bulb surface, the bulb surface at the valve tips, and bulb terminus were easy to consistently identify and compare [NO] between 12 bulbs in 9 animals. The inset tracing of a lymphatic bulb image identifies these regions. The tips of the valves were associated with the highest bulb surface [NO], and even the origin of the valves raised the [NO] relative to the bulb origin and terminus. The [NO] from valve tip regions to the terminus of bulbs decreased dramatically, and the terminal [NO] were only marginally higher than the origin surface [NO]. *Significant difference from the bulb origin.