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. 2011 Aug 5;301(5):L782–L788. doi: 10.1152/ajplung.00079.2011

Fig. 2.

Fig. 2.

A: agarose gel showing HA fragmentation. Duplicate left lung samples were run without or with hyaluronan lyase (HYAL) to quantify the amount of HA fragments present. Left lungs were harvested immediately after LPAL (0 h) and 4 h after LPAL. Brackets next to the HA ladder indicate the size range where pixel intensities were evaluated. B: agarose gel showing HA fragmentation as in A. Left lungs were harvested 4 h after LPAL from mice without or with N-acetyl-l-cysteine (NAC) pretreatment. C: comparison of average changes in HA fragments in 3 size ranges (495–310 kDa, 310–110 kDa, and 110–30 kDa), 0 h and 4 h after LPAL (n = 7–10 mice/group). A significant increase in fragmentation occurred by 4 h after LPAL (*P < 0. 5 from 0 h). Fragmentation in lungs from NAC-treated mice was substantially reduced compared with untreated mice 4 h after LPAL within the ranges 495–310 kDa and 310–110 kDa (#P < 0.05 from 4 h).