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. 2004 Feb;78(3):1488–1502. doi: 10.1128/JVI.78.3.1488-1502.2004

FIG. 10.

FIG. 10.

Immunoblot analysis of UL99 deletion mutant virion proteins with the HCMV VP antibody. Virions were enriched by sucrose gradient centrifugation from the extracellular medium of infected HFF cells. (A) RV17179 expresses the resident UL99 protein truncated after amino acid 99; RV18074 is a rescued derivative of RV17179 that expresses wild-type UL99. (B) RV19029 and RV19030 express UL99 proteins truncated after amino acid 57, either in the absence or in the presence of the wild-type UL99 protein, respectively.