Fig. 1.
Acute S-nitrosoglutathione (GSNO) repletion alters airway hyperresponsivity in the ovalbumin-sensitized and challenged (OVA) mouse. A: schematic showing experimental protocol. B and C: average (Ave) total pulmonary resistance (RT) in alum control or OVA, tracheotomized, and mechanically ventilated mice was measured by flexiVent immediately after a 15-s dose of aerosolized PBS or 1–100 mM GSNO via ultrasonic nebulizer followed by methacholine (MCh) challenge. Data in C are area under the curve calculated using mean RT at 0 mM MCh as a baseline within each treatment group. D: total S-nitrosothiols (SNOs) in bronchoalveolar lavage (BAL) from PBS- or GSNO-treated mice were quantified by mercury-coupled photolysis chemiluminescence. i.p., Intraperitoneal. Data in B and C are means ± SE (n = 5–11 per group) and in D are means ± SE (n = 5 per group). *P < 0.02, **P < 0.001.