Fig. 3.

Representative Western blots of proteins in adipocyte LD and concanavalin-A (ConA)-resolved LD fractions. Western blots loaded with samples from adipocyte LD homogenates (lane 1), nonbinding LD (lane 2), perilipin-enriched LD (lane 3), and protein standards (lane 4) were probed against the antibodies anti-perilipin (A), anti-ADRP (B), anti-TIP47 (C), anti-caveolin-1 (D), anti-flotillin (E), anti-hormone-sensitive lipase (HSL; F), anti-adipose triglyceride lipase (ATGL; G), and anti-comparative gene identification-58 (CGI58; H), as described in materials and methods. The protein standard was detected in homogenates of mouse adipose tissue.