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. 2011 Aug 16;301(5):E991–E1003. doi: 10.1152/ajpendo.00109.2011

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Fig. 4. — Immunoprecipitation of proteins in ConA-resolved LD fractions. Perilipin-enriched LD fractions (A and B) and unfractionated LD (C and D) were immunoprecipitated (IP) with antibodies specific for perilipin (A), caveolin (B), ADRP (C), or caveolin (D) using the Catch and Release version 2.0 system, following the manufacturer's protocol. IP with a negative control of rabbit IgG (lane 2 in A–D) was performed to assess nonspecific binding. Samples were then analyzed by Western blot analysis, immunoblotted (IB) with caveolin (A), perilipin (B), caveolin (C), or ADRP (D), respectively.