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. 1999 Feb;119(2):627–634. doi: 10.1104/pp.119.2.627

Figure 1.

Figure 1

Growth at different pH levels of yeast cells expressing wild-type and mutant plant plasma membrane H+-ATPases. A, The wild-type YPS14-4 strain (yeast PMA1 under its own promoter), the recipient strain YAK2 (yeast PMA1 under the GAL1 promoter), YAKpma2, YAKpma4, and the mutants T861N and Q882ochre were grown in YGlu medium at pH 6.5 and spotted onto solid YGlu medium at pH 3.0, 4.0, 5.0, 6.0, and 7.0, and at pH 5.5 in the presence of 20 mm acetic acid (HAc). B, The indicated strains were inoculated in liquid YGlu medium at pH 4.0, 5.0, 6.0, and 7.0, and the cell number was scored periodically. The duplication time was calculated during the exponential phase. ND, Not determined; ∞, no division.