Fig. 2.

Diurnal profiles of oxytocin (OXT) release in chow-fed and HFD-fed mice. Adult male C57BL/6 mice received HFD vs. chow feeding from an adult age (∼10 wk) for 12 wk and then were measured for 24-h profile of serum OXT levels (A) or ex vivo OXT release under basal and depolarization (KCl) conditions as described in materials and methods (B). *P < 0.05; n = 8–10 (A) and n = 6–7 (B) mice/group. Error bars represent means ± SE. Statistics in A indicate the comparisons between HFD-fed mice and chow-fed mice at the matched time points.