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. 2004 Feb;78(3):1448–1455. doi: 10.1128/JVI.78.3.1448-1455.2004

FIG. 2.

FIG. 2.

siRNA silencing of CD81 expression in Huh-7.5 cells inhibits HCV pseudotype infection. (A) Huh-7.5 cells transfected with either siIRR or siCD81 were stained with IgG1 or anti-CD81 MAb (1.3.3.22) at 54 h posttransfection and analyzed by flow cytometry. (B) siRNA-transfected Huh-7.5 cells were infected with the indicated pseudotypes and analyzed for luciferase activity. (C) siRNA-transfected Huh-7.5 cells were infected similarly with the indicated range of HIV-HCV H pseudotype inocula and analyzed for luciferase activity. All infections were performed in triplicate, and the mean luciferase activity is shown.