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. 2004 Feb;78(3):1448–1455. doi: 10.1128/JVI.78.3.1448-1455.2004

FIG. 3.

FIG. 3.

Expression of CD81 in HepG2 and HH29 cells confers susceptibility to HCV pseudotype infection. (A) HepG2, HH29, and Huh-7.5 cells alone (upper panels) or after transduction with TRIP-CD81 (lower panels) were stained with an irrelevant IgG and anti-CD81 MAb (1.3.3.22) and analyzed for CD81 expression by flow cytometry. Quadrants were established based on irrelevant isotype-matched IgG staining of the cells. FSC, forward scatter. (B) Transduced (shaded bars) and nontransduced (hatched bars) HepG2, HH29, and Huh-7.5 cells were infected with the indicated pseudotypes 48 h posttransduction and analyzed for luciferase activity. (C) Transduced cells were incubated with either MAbs specific for CD81 (5A6) or an irrelevant isotype-matched IgG at 5 μg/ml and infected with HIV-HCV H E1E2 or with HIV-HCV H E1E2 preincubated with anti-E2 MAb 11/20. All infections were performed in triplicate, and the mean luciferase activity is shown.