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. 2011 Nov 11;6(11):e26809. doi: 10.1371/journal.pone.0026809

Figure 4. PLCβ activity is not required for AmDOP2- or AmOA1-mediated intracellular cAMP signaling.

Figure 4

Treatment with edelfosine (edlf) had no significant effect on [cAMP]i signaling mediated via AmDOP2 receptors (P = 0.9751), or via AmOA1 receptors (P = 0.2224). HEK293 cells were co-transfected with expression constructs for either the AmDOP2 receptor (A) or AmOA1 receptor (B), and a CRE-luciferase reporter construct. Cells were treated with either 1 µM agonist (DA and OA respectively) or 1 µM agonist and 10 µM edelfosine (edlf). Data are normalized to the response observed in cells not treated with edelfosine, and the mean of three independent experiments within which, each treatment was tested twice. Error bars represent the SEM. Statistical significance was determined using Student's two-tailed t tests.