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(A) Procyclic-form 29-13 cells harboring pLew100-TbPlk-EYFP or pLew100-Cdc5-EYFP were cultured for 1 day in the presence of 1.0 µg/ml tetracycline, fixed with 3.7% formaldehyde, and stained with DAPI. (B) The cytoskeletons were stained with L3B2 antibody for FAZ (red), and DAPI for DNA (blue). The results were from three independent experiments. Arrows indicate the TbPlk-EYFP and Cdc5-EYFP signals. Bars: 2 µm.
