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. 2011 Nov 11;6(11):e27265. doi: 10.1371/journal.pone.0027265

Figure 1. Effect of scopolamine on BDNF and GFAP expression in mice brain.

Figure 1

Dose and time dependent effects of scopolamine (SC- 1, 3, 6, 10 mg/Kg BW) on the level of expression of BDNF and GFAP mRNA in mouse cerebrum: (A) RT-PCR analysis showing BDNF (692 bp), GFAP mRNA (641 bp) and internal control GAPDH (108 bp). The band intensity was quantitated densitometrically (represented as relative units) by normalizing with GAPDH) from three independent experiments (±) SEM. (B) Histogram represents percent relative units of BDNF and GFAP expressions from three independent experiments (±) SEM. *denotes significant differences (P<0.05) between the groups. (C) Time (1, 3, 6, 24 h) dependent effect of scopolamine (SC- 3 mg/Kg BW) on expression of BDNF and GFAP mRNA in mouse cerebrum. Data was quantitated (D) as mentioned above.