Figure 7. Treatment with Ashwagandha i-Extract/withanone and protection against scopolamine-induced DNA damage and oxidative stress.

Level of expression of DNA damage marker (γH2AX) (A) and oxidative stress marker (reactive oxygen species, ROS) (B) in C6 cells treated with either scopolamine or scopolamine and i-Extract/withaferin A/withanone as detected by immunocytochemistry with specific antibodies and as described in material and methods are shown. Quantitation of γH2AX (C) and ROS (D) are shown, in which 200–300 cells were counted and scored with high intensity of signal.