Table 1. Gene specific primer sequences and corresponding PCR conditions.

Gene name/Amplicon length	Primer Sequences		Annealing conditions			Number of cycles
			Denature	Anneal	Extend
GAPDH/108 bp	Forward	5′ GTCTCCTGCGACTTCAG-3′	94°C 30 s	52°C 30 s	72°C 30 s	26
	Reverse	5′-TCATTGTCATACCAGGAAATGAGC-3′
GFAP/641 bp	Forward	5′-TTCCTGTACAGACTTCTCC-3′	94°C 60 s	52°C 30 s	72°C 45 s	29
	Reverse	5′-CCCTTCAGGACTGCCTTAGT-3′
BDNF/692 bp	Forward	5′-TGCCAGAGCCCCAGGTGTGA-3′	94°C 60 s	63°C 30 s	72°C 45 s	32
	Reverse	5′-CTGCCCTGGGCCCATTCACG-3′