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. 2011 Nov 11;6(11):e27302. doi: 10.1371/journal.pone.0027302

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Heins et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Western blot using anti-BLA antibodies of the soluble fraction of cells expressing MBP317-347, Ph8 and sucrose switch 5–7 (arrow, ∼70 kDa). Cells were cultured with maltose (mal), sucrose (suc), or neither sugar (−). (B) Correlation between the MIC_Amp and the concentration of maltose added to the culture for cell expressing MBP317-347 (•) or MBP317-347(W340A) (□). (C) Accumulation levels dictate the MIC_Amp. Correlation between the accumulation ratio (+maltose/−maltose), as measured by western blot, and the MIC_Amp ratio (+maltose/−maltose) of cell expressing MBP317-347 (•) or MBP317-347(W340A) (□). Different accumulation ratios were obtained by culturing the cells in the presence of different concentrations of maltose.