Table 2. The effect of maltose on MIC_Amp and protein expression.

	MICAmp (µg/ml)b			Accumulationc
Proteina	− Maltose	+ Maltose	Ratiod	Ratiod	Stdeve
Nonef	1	1	1	2.4	0.2
MBP	1	1	1	1.0	0.1
BLA	8192	8192	1	1.3	0.1
MBP317-347	16–32	512	16–32	31.3	4.5
Ph1	32	512–1024	32–64	14.6	3.1
Ph3	256	1024–2048	4–8	10.7	1.0
Ph5	16–32	256–512	8–32	17.6	5.9
Ph7	32–64	1024	16–32	25.7	6.5
Ph8	32	512–1024	16–32	19.2	7.5
Ph12	64	1024–2048	16–32	38.9	10.1
Ph14	128	2048	16	12.4	6.8
Ph16	1024	4096	4	5.4	2.1
Ph17	32	1024	32	49.2	7.9
Ph19	256	2048	8	14.7	2.2
Ph24	4–8	128	32	21.5	4.7
Ph27	256	2048	8	11.1	3.7
Ph28	64	1024–2048	16–32	22.7	7.2
c4	1024–2048	2048	1–2	5.3	5.0

^aProtein expressed from plasmid pDIMC8 in RH22 cells.

^bMIC_Amp was determined in triplicate. For proteins with a MIC_Amp range, this indicates the range from the three experiments (e.g. Ph5 was found to have a MIC_Amp in the absence of maltose of 16 µg/ml in some trials and 32 µg/ml in other trials).

^cAccumulation ratio determined from quantitative image analysis of western blots probed using polyclonal anti-BLA antibodies (except for MBP which was probed with anti-MBP antibodies).

^d(With maltose)/(without maltose).

^eStandard deviation calculated from three independent experiments.

^fNo pDIMC8 plasmid was present. The protein monitored by western blot was chromosomally encoded MBP.