FIG. 9.

Intracellular localization of VZV IE63 and gE in cells infected with IE63 mutant viruses. Melanoma cells were infected with rOKA/ORF63rev (1 a,b; 5 a,b), rOKA/ORF63rev[T171] (2 a,b), rOKA/ORF63rev[T181] (3 a.b), or rOKA/ORF63rev[S185] (4 a, b). Monolayers were stained with anti-rabbit IE63 antiserum (red) and counterstained with the nuclear marker, 4′,6′-diamidino-2-phenylindole (DAPI) (blue) (panel 5 a, b) or with anti-gE monoclonal antibody (fluorescein isothiocyanate: green) (panels 1 to 4) and counterstained with the nuclear marker DAPI (blue). Panels 2a to 4a, arrows indicate abnormal, irregular polykaryocytes with punctate distribution of gE. Immunofluorescence microscopy was performed at 4 days after infection (late). Magnification, 40×.