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. 2011 Nov 11;6(11):e27478. doi: 10.1371/journal.pone.0027478

Figure 6. Nedd4 facilitates lysosomal sorting of LAPTM4a and LAPTM4b.

Figure 6

(A) WT or Nedd4−/− MEF cells were transfected with (wildtype) mCh-LAPTM4a or mCh-LAPTM4b (red). 24 hrs post transfection the plasma membranes was stained (ConA, blue), cells were fixed and incubated with anti-Lamp1 antibodies (green). Cells were imaged by confocal microscopy and subcellular localization was assessed using Volocity 5.4.1. (B) LAPTM4 colocalization with the lysosomal marker Lamp1 is expressed in terms of the Pearson's correlation coefficient. Degrees of freedom are noted as (r), level of significance as (p). (C) The graphs represent the changes in mean Pearson's coefficients of WT or Nedd4−/− MEF cells expressing mCh-LAPTM4a (n = 26) or mCh-LAPTM4b (n = 30). Error bars indicate SEM and * indicates p<0.0001.