FIG. 3.

The E5 peptide 25-33 (VCLLIRPLL) is the CTL epitope in C57BL/6 mice. CTL activity among these four peptides of pool II (peptides 20-28, 25-33, 30-38, and 35-43) was measured by analyzing CD8⁺ IFN-γ⁺ double-positive cells from rAd-E5-vaccinated mice as described in Materials and Methods. (A) Splenocytes from vaccinated mice were stimulated in vitro with each peptide and stained for both CD8⁺ and IFN-γ⁺ antibodies. The result of one representative assay from five similar independent experiments is shown. (B) Summary of the five independent experiments. The data represent the means and standard errors of five experiments. The y axis shows (the number of E5-specific CD8⁺ IFN-γ⁺ T cells/the total number of tested lymphocytes) × 100%.