FIG. 4.

Screening the CTL activity of the potential K^b- and D^b-binding peptides of HPV-16 E5. The splenocytes from rAd-E5 vaccinated C57BL/6 mice were harvested and stimulated with each E5 peptide, and then intracellular cytokine staining with flow cytometry was performed to determine the number of CD8⁺ IFN-γ⁺ double-positive cells as described for Fig. 2. The data represent the means and standard error of five experiments. The x axis denotes the fold increase of E5-specific splenocytes, calculated as follows: (the number of vaccinated splenocytes stimulated with peptide 25-33/the number of vaccinated splenocytes stimulated with irrelevant peptide) × 100%.