Figure 5. Effect of km concentration, copy number of the hemolytic plasmid and growth temperature on growth rate and generation of Hly- clones.
A. Growth curves of the strain MG1655HY (pANN202-312R) in LB medium containing either no km (
), 12.5 mg/l km (
) or 25 mg/l km (
). B. Growth curves of the strain MG1655HY (pHly152) in LB medium containing either no km (
) or 25 mg/l km (
). C. Growth curves of strain MG1655HY (pANN202-312R) in LB medium at 25 (
), 30 (
) or 37°C (
). Hly- derivatives were only detected in the culture of strain MG1655HY (pANN202-312R) grown in LB medium containing 25 mg/l km.