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. 2011 Nov 11;6(11):e27606. doi: 10.1371/journal.pone.0027606

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Pedró et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Growth curves of strain MG1655 (pHly152) in LB medium containing no km (), 5 () and 10 () mg/l of km. B. The percentage of Hly^- clones in the culture grown in LB km (5 mg/l). C. PCR analysis using primers PANR8 and NBM18 to confirm the presence of pHly152 in seven Hly^- clones (lanes 2 to 8). Lane 1, molecular mass marker. Lane 9, negative control (strain MG1655). Lane 10, positive control (strain MG1655 (pHly152)). D. PCR analysis to confirm deletions 1 and 2 in the Hly^- clones. Primers used were PANR1 and PANR6, and the fragments amplified were of 875 bp and 2126 bp for the deletions 1 and 2 respectively.

Inline graphic — A. Growth curves of strain MG1655 (pHly152) in LB medium containing no km (), 5 () and 10 () mg/l of km. B. The percentage of Hly^- clones in the culture grown in LB km (5 mg/l). C. PCR analysis using primers PANR8 and NBM18 to confirm the presence of pHly152 in seven Hly^- clones (lanes 2 to 8). Lane 1, molecular mass marker. Lane 9, negative control (strain MG1655). Lane 10, positive control (strain MG1655 (pHly152)). D. PCR analysis to confirm deletions 1 and 2 in the Hly^- clones. Primers used were PANR1 and PANR6, and the fragments amplified were of 875 bp and 2126 bp for the deletions 1 and 2 respectively.