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. 2011 Nov 11;6(11):e27606. doi: 10.1371/journal.pone.0027606

Figure 7. Deletion of the hly operon in strain MG1655 (pHly152) growing with a subinhibitory ampicillin concentration.

Figure 7

Analysis of seven Hly- clones isolated from strain MG1655 (pHly152) grown in LB medium containing ap (10 mg/l). A. PCR analysis using primers PANR8 and NBM18 to confirm the presence of pHly152. B. PCR analysis to confirm deletions 1 and 2 in the Hly- clones. Primers used were PANR1 and PANR6, and the fragments amplified were of 875 bp and 2126 bp for the deletions 1 and 2 respectively.