Table 1. Oligonucleotides used in this study.
Name | Sequence (5′ 3′) | Purpose |
PANR8 | TCTGCGTGGAAGTATGAGC | To determine the presence/absence of pHly152 |
NBM18 | CCATGCTGATGTGGCGCTTA | |
PANR1 | GATGATGCCACAAAATGGAT | To determine which recombination took place |
PANR6 | TTCCTGACACAGAACCGTAA | |
NBM1 | ACTCAGGAAACCGTAGTACCT | To sequence pANN202-312R |
NBM3 | GTAAAAGCCGCGAGGACAACG | |
NBM4 | GAAGCCAAGTCAAACAACAG | |
NBM5 | GCTGCTTCCTTCAACTGCCA | |
NBM6 | GGATTGCGCACCGGAAACCC | |
NBM8 | GATGCTTCCCCGTTTTGCCG | |
NBM12 | TATGACGCCACCCTACCAGT | |
NBM14 | CATTGCCATTGAAGCGGAGC | |
RECAH1 | CAGAACATATTGACTATCCGGTATTACCCGGCATGACAGGAGTAAAAATGGTGTAGGCTGGAGCTGCTTC | To construct recA mutants |
RECAH2 | ATGCGACCCTTGTGTTACAAACAAGACGATTAAAAATTTCGTTAGTTTCCATATGAATATCCTCCTTAGT | |
RECAUP | CTTGTGGCAACAATTTCTACA | To check for recA mutation |
RECADOWN | TCATGGCATATCCTTACAACT | |
RPOSUP | TGCCGCAGCGATAAATCG | To check for rpoS mutation |
RPOSDOWN | CGGACCTTTTATTGTGCACAG |