Figure 6. Molecular PrP^res typing of atypical scrapie affected tg338 mice.

Western blots for PrP^res of representative brain extracts following SDS-PAGE using (A) 16.5% tris-glycine and (B-F) 4–20% tris-glycine gradient gels. Immunochemical detection was performed with antibodies P4, Sha31 or 9A2 as indicated. In some instances (D, E), samples were deglycosylated with PNGaseF. (F) Atypical scrapie PrP^res banding pattern following sample pretreatment with different concentrations of proteinase K (PK, in µg/ml), compared to the PK digestion protocol of the Bio-Rad TeSeE confirmatory Western blot (BR). Identification codes for the atypical scrapie isolates are shown at the top. Controls: cl, classical scrapie affected tg338 mouse; non-inoc, non-inoculated tg 338 mouse; neg s, TSE negative sheep. Molecular mass standards in kDa and loaded tissue equivalents are indicated.