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. 2011 Nov 11;6(11):e27666. doi: 10.1371/journal.pone.0027666

Figure 7. Antigen-specific IFN-γ CBA responses by human PBMC stimulated with IVTT-proteins.

Figure 7

PBMCs were cultured with IVTT-produced FluM, FluHA, CMVpp65 and EBNA3A purified using (A) NI-NTA nickel resin or (B) MagneHis Ni-particles; (C) unpurified; associated to (D) Polybeads or (E) ProteinG beads; diluted 1∶100, 1∶1000 or 1∶10,000. Negative controls were medium only, unpurified empty pIVEXHisHA IVTT extract, and empty pIVEXHisHA IVTT extract associated to polybeads or protein G beads. Positive controls were CEF peptide pool or PHA (data not shown). Secreted IFN-γ in the supernatant of cultured PBMCs was analysed by Cytometric Bead Array after 72 hrs stimulation. * P<0.05 compared to negative controls.