FIG. 3.

Accumulation of RNAs with mutations near the initiation site in BMV plus-strand RNA3 template. (A) Schematic of the 3′ 13 nucleotides of minus-strand BMV RNA3 that direct plus-strand RNA3 synthesis. The initiation cytidylate is in bold, and the numbers represent the position relative to the initiation site. Arrows point to the substitutions made at each nucleotide. (B) Northern blot analysis of progeny RNA accumulated in barley protoplasts at 21 h posttransfection. The mutated RNAs named above the lanes in the autoradiogram were transfected into protoplasts, and the progeny RNAs were processed for minus-strand and plus-strand BMV RNA accumulation. The 18S rRNA accumulation is shown to establish that similar amounts of RNAs were loaded in each lane.