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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cancer Res. 2011 Sep 9;71(21):6848–6856. doi: 10.1158/0008-5472.CAN-11-1745

Figure 4. Expression of ccRCC markers in γ-HIF1α-M3-43 kidneys.

Figure 4

Representative images of immunostaining using CD31 (A,B), CA-IX (C,D), Glut-1 (E,F), and VEGF antibodies (G,H) in 6 month γ-HIF1α-M3-43 TG+ PTs (A, C, E, G) and TG- PTs (B, D, F, H). Increased staining of CD31 (A), CA-IX (C), Glut-1 (E), and VEGF (G) is only observed in the abnormal PTs of TG+ mice. Arrow (panel A), strong CD31 staining (red) around PT. Nuclei stained with DAPI (blue). Arrow (panel C), cup-like CA-IX staining of basal and lateral membranes of “clear” cells. Arrow (panel E), strong Glut-1 staining around empty vacuole in “clear” cells. Arrow (panel G), strong VEGF staining around empty vacuole in “clear” cells. Representative Western Blot (I) of CA-IX protein and RT-PCR (J) of CA-IX and CD70 mRNA and β-Actin (I,J), control. Scale bars, 100 μm.