Figure 8.

Immunoblot analyses showing the expression of PDH subunits from various organs. Maize kernels were allowed to soak for 2 d prior to isolating the endosperm and scutellum. Etiolated shoots were grown for 5 d in complete darkness. The remaining samples were obtained from light-grown plants, grown in either a growth chamber (four-leaf stage) or a greenhouse (adult). As a control, the blots were reprobed with antibodies specific to other mitochondrial proteins, HSP70 and the β-subunit to ATP synthase. Approximately 25 μg of a total protein preparation was loaded per lane. Molecular masses of polypeptides are indicated on the left (in kilodaltons).