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Determination of successful membrane targeting of prestin mutations. A: typical human embryonic kidney (HEK) cell expressing prestin-enhanced green fluorescent protein (eGFP) (green) and labeled with WGA-633 (red). Inset: fluorescence intensity profiles of the line in the main image. B: average (+SE) of separation of WGA-633 and prestin in profiles, as in A, for wild-type (WT) and mutated prestins as indicated.
