Table 1.

Averaged V_pk and z values of prestin NLC of wild-type prestin and prestin mutations, their statistically significant differences from wild type in the same batch, and their conservation group status

Conserved	Mutation	Vpk, mV	P Value	z	P Value
Group 1	C52A
	Control	−48.33		0.901
	Test	−58.45	0.051	0.902	0.986
Group 2	C196A
	Control	−52.38		0.809
	Test	−54.71	0.615	0.918	0.082
	C381A
	Control	−50.25		0.913
	Test	−59.34	0.211	0.931	0.907
	C679A
	Control	−53.48		0.918
	Test	−45.25	0.055	0.936	0.740
Group 3	C124A
	Control	−49.94		0.923
	Test	−72.78	<0.001	0.944	0.849
	C192A
	Control	−52.23		0.817
	Test	−63.05	0.006	0.954	0.077
	C260A
	Control	−50.98		0.861
	Test	−60.22	0.025	0.882	0.766
	C395A
	Control	−59.34		0.850
	Test	−51.41	0.053	0.797	0.438
	C415A
	Control	−53.74		0.944
	Test	−32.11	<0.001	0.937	0.928
Group 3	C124S
	Control	−52.03		0.866
	Test	−67.65	0.007	0.997	0.124
	C415S
	Control	−47.62		0.949
	Test	−59.26	0.132	0.821	0.139

Values are averaged peak membrane potential (V_pk) and charge transfer (z) for prestin nonlinear capacitance (NLC) of wild-type prestin (control) and prestin mutations. See text for descriptions of conservation groups 1–3.