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Horizontal brain stem slices from rat embryos. A–C: brightfield images of brain stem slices at embryonic day 14 (E14), E16, and E18. The trajectory of solitary tract (ST) is indicated by asterisks. Approximate recording sites are circled by dots. B and C inset: 1, 1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate anterograde transport from the geniculate ganglion to illustrate collateral, medially directed branching of the ST.
