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. 2004 Feb;78(3):1109–1120. doi: 10.1128/JVI.78.3.1109-1120.2004

FIG. 7.

FIG. 7.

Endocytosis and lysosomal degradation of MHC-I by MARCH-IV and MARCH-IX. (A) Uptake of MHC-I in HeLa cells transfected with vector control, MARCH-IV or MARCH-IX. At 24 h posttransfection, MHC-I was stained with antiserum K455 and either fixed after 30 min at 4°C (top panel) or transferred to 37°C for 2 h (bottom panel) prior to fixation and staining with Alexafluor 594 anti-rabbit secondary antibody. (B) Flow cytometry of MHC-I (W6/32 staining) on MARCH-IV or IX-transfected HeLa cells in the presence or absence of concanamycin A.