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Flow cytometry determination of the S-phase distribution of asynchronously growing Huh7 2-3c cells (A) and clonally related 2-3 cells (B) containing replicating, full-length HCV RNA. Cells were labeled with BrdU for 30 min and then analyzed by flow cytometry after being stained for BrdU incorporation and DNA content. The percentage of BrdU-positive cells in the two cell lines is indicated.
