FIG. 5.

Flow cytometry analysis of cell cycle entry in populations of Huh7 2-3c cells (A) and clonally related 2-3 cells (B) containing full-length, replicating NNeo/C5B RNA. Cells were arrested in the G₁ phase of the cell cycle with aphidicolin and released from the cell cycle block after 24 h. Prior to harvesting at the indicated time points, cells were labeled with BrdU for 30 min. BrdU incorporated into S-phase cells was stained with a monoclonal FITC-conjugated antibody, and the total DNA content was determined by staining with 7-AAD.