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Immunofluorescence 24 h after NP-mediated transfection. pIRES-hrGFP-HMGB1 transfection with NP-mediated protocols. Plano-AuNP (a, b, c), PLAL-AuNP Size 2 (d, e, f), and MA Lipofection (g, h, i)). Primary antibody: mouse anti-HMGB; secondary antibody: goat anti-mouse (Texas Red fluorochrome). a, d, g: GFP and Red Fluorescence merged image; b, e, h: GFP Fluorescence and (c, f, i) Red Fluorescence images. Scale bar 75 μm.
