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Detection of US3 in cells infected with tegument protein deletion mutants of PrV. Rabbit kidney cells infected with PrV-ΔUL37 (A and B), PrV-ΔUL47 (C and D), PrV-ΔUL48 (E and F), PrV-ΔUL3.5 (G and H), PrV-ΔUL11 (I and J), and PrV-gEIM− (K and L) were analyzed by conventional electron microscopy (A, C, E, G, I, and K) or immunoelectron microscopy with a monospecific anti-US3 serum (B, D, F, H, J, and L). Bars: 500 nm (A, C, E, G, I, and K); 250 nm (B, D, F, H, J, and L).
