FIG. 2.

Transcriptional response assays of AML1 and its unphosphorylated mutant in the presence of TSA. P19 cells were cotransfected with 500 ng of Tww-tk-Luc, 200 ng of pCMVMK, and 20 ng of pME18S AML1, −), pME-AML1 (AML1, wild type), or pME-S249/266A as indicated with (B) or without (A) treatment with TSA. The cells were cultured for 30 to 36 h after transfection and harvested. The means and standard deviations of the luciferase activities of AML1 and S249/266A in two independent transfections are shown. We used the mean of the luciferase activities of the expression vector (pME18S) in two independent transfections as the control. Similar results were obtained in four additional independent transfections.