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. 2004 Feb;24(3):1033–1043. doi: 10.1128/MCB.24.3.1033-1043.2004

FIG. 3.

FIG. 3.

Intranuclear translocation of AML1 induced by its phosphorylation. (A to F) COS-7 cells were transfected with FLAG-tagged wild-type AML1, together with ERK and PEBP2β, and incubated in the absence (A to C) or the presence (D to F) of EGF. The cells were treated with in situ nuclear-matrix preparations, and immunofluorescent staining was performed using the anti-FLAG antibody (A and D), the anti-mSin3A antibody (B and E), or both (C and F). (G to L) COS-7 cells were cotransfected with FLAG-tagged S249/266A (G to I) or S249/266E (J to L) with PEBP2β. The cells were treated with in situ nuclear matrix preparations, and immunofluorescent staining was performed using the anti-FLAG antibody (G and J), the anti-mSin3A antibody (H and K), or both (I and L).