FIG. 6.

Effect of a mutation in the nuclear-matrix attachment site of AML1 on interaction with mSin3A. COS-7 cells were transfected with pME-FLAG-AML1 (lane 1) or pME-FLAG-AML1 1-288 (lane 2) and harvested. The cell lysates were precleared with protein G-Sepharose, mixed with anti-mSin3A antibody, and rotated for 2 h at 4°C. Then, mSin3A was recovered on protein G-Sepharose beads. The washed beads were subjected to SDS-PAGE, followed by Western blotting with anti-FLAG or anti-mSin3A antibody. Expression of each protein was confirmed by using 30 μg of total lysates. Arrows, wild-type and mutant AML1.