Table 1.
Exemplary table for the purification of MroAPO by fast protein liquid chromatography (FPLC)
| Steps | Total Activity (Units) |
Total Protein (mg) |
Specific Activity (U mg-1) |
Yield (%) |
Purification (fold) |
|---|---|---|---|---|---|
| Culture fluid | 11,700 | 730 | 16 | 100 | 1 |
| Ultrafiltration step | 11,520 | 415 | 28 | 98 | 1.7 |
| Q Sepharose FF | 10,413 | 218 | 48 | 89 | 3.0 |
| Mono Q | 6,235 | 118 | 53 | 53 | 3.3 |
| Source 15Q | 1,457 | 22 | 88 | 17 | 5.5 |
| SEC | 768 | 9 | 90 | 7 | 5.6 |
| Mono Q | 75 | 0.98 | 76 | 0.7 | 4.8 |
Enzyme activities are based on the oxidation of veratryl alcohol to veratrylaldehyde at pH 5.5. The purification processes started with 468 mL culture fluid from the bioreactor fermentation (volume activity 25 U mL-1) corresponding to a total of 11,700 Units.