Table 3.
Apparent kinetic parameters of purified MroAPO
| Substrate | pH |
Km (mM) |
kcat (s-1) |
kcat/Km (s-1 M-1) |
|---|---|---|---|---|
| ABTS | 4.5 | 0.071 | 25 | 3,53*105 |
| DMP | 5.5 | 0.133 | 70 | 5.29*105 |
| Benzyl alcohol | 5.5 | 0.118 | 62 | 5.29*105 |
| Veratryl alcohol | 5.5 | 0.279 | 49 | 1.76*105 |
| Naphthalene | 5.5 | 0.791 | 33 | 4.25*104 |
| H2O2 | 5.5 | 3.14 | 76 | 2.42*104 |
Reactions were performed in sodium phosphate/citrate buffer (pH 4.5 or 5.5) in the presence of 2 mM H2O2 at 25°C. The Km for the co-substrate H2O2 was determined in the presence of 5 mM veratryl alcohol. The data are means of three parallel experiments with standard deviation < 5%.