FIG. 4.

Putative NF-κB binding sites in the PTEN promoter do not mediate repression by p65. NIH 3T3 cells were cotransfected with either full-length PTEN promoter-Luc reporter construct or Δ600 PTEN promoter-Luc reporter construct, in which the two putative NF-κB binding sites were deleted, along with either vector or p65 constructs, and luciferase activity was determined at 48 h posttransfection (A). NIH 3T3 cells were cotransfected with either empty Tal-Luc reporter construct or PTEN 600 Tal-Luc reporter construct, which contained the 600-bp region of the PTEN promoter with the two putative NF-κB binding sites subcloned in Tal-Luc, along with either vector or p65 constructs, and luciferase activity was determined at 48 h posttransfection (B). Relative Luc activity, normalized to the corresponding β-galactosidase activity, is shown (A and B).