Table 1.
Strains and plasmids used in this study
| Strain/Plasmid | Genotype | Source |
|---|---|---|
| Strain | ||
| E. coli BW25113 | lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | [34] |
| E. coli DH5α | luxS supE44 ΔlacU169(Φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | [21,35] |
| E. coli DH5α/pMMB206/pRK2013 | E. coli harboring pMMB206 and pRK2013 | This study |
| E. coli DH5α/pMMB206-BdcA/pRK2013 | E. coli harboring pMMB206-BdcA and pRK2013 | This study |
| P. aeruginosa PA14 | P. aeruginosa wild-type strain | [36] |
| P. fluorescens | P. fluorescens wild-type strain | R. Frazee |
| R. meliloti 102F34 | R. meliloti wild-type strain | M. Sadowski |
| PA14/pMMB206 | P. aeruginosa wild-type harboring pMMB206 | This study |
| PA14/pMMB206-BdcA | P. aeruginosa wild-type harboring pMMB206-BdcA | This study |
| PA14/pMMB206/pP25-gfp | P. aeruginosa wild-type harboring pMMB206 and pP25-gfp | This study |
| PA14/pMMB206-BdcA/pP25-gfp | P. aeruginosa wild-type harboring pMMB206-BdcA and pP25-gfp | This study |
| P. fluorescens/pMMB206 | P. fluorescens wild-type harboring pMMB206 | This study |
| P. fluorescens/pMMB206-BdcA | P. fluorescens wild-type harboring pMMB206-BdcA | This study |
| R. meliloti 102F34/pMMB206 | R. meliloti wild-type harboring pMMB206 | This study |
| R. meliloti 102F34/pMMB206-BdcA | R. meliloti wild-type harboring pMMB206-BdcA | This study |
| R. meliloti 102F34/pMMB206/pP25-gfp | R. meliloti wild-type harboring pMMB206 and pP25-gfp | This study |
| R. meliloti 102F34/pMMB206-BdcA/pP25-gfp | R. meliloti wild-type harboring the pMMB206-BdcA and pP25-gfp | This study |
| Plasmid | ||
| pMMB206 | Cmr, Mob+, lacIq, broad-host range plasmid | [23] |
| pMMB206-BdcA | Cmr; pMMB206 with Ptac-lacUV5::bdcA | this work |
| pRK2013 | Kmr; Tra+ Mob+ (RK2) Km::Tn7 ColEl origin, helper plasmid for mobilization | M. Bagdasarian, [37] |
| pP25-gfp | Carr; constitutive green fluorescent protein production | [27] |
Cmr, Carr, and Kmr, denote chloramphenicol, carbenicillin, and kanamycin resistance, respectively.