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PCR primers used to isolate Phr1 cDNA
| Primer pair | Product size (bp) | Sequence
|
|
|---|---|---|---|
| Forward | Reverse | ||
| Phr1.F1.2-Phr1.R1.1 | 2,641 | GCAGCCGCCACCATCTCTTCC | GCTGAGCTGGATGGGATCTGAG |
| Phr1.F2-Phr1.R2 | 3,000 | GTTGCAAGGCATGTGCAAGAG | GCTATGTCACTGGGTGAATCG |
| Phr1.F3-Phr1.R3 | 3,792 | GGAGGTGTTGGTTGATGATAGTG | CCTTCATCCACACTCTTCAAGTC |
| Phr1.F4-Phr1.R4 | 2,204 | GTGAGGTAGTTGAAGTCTGTAC | CATCATAAGGTCAGAGATGGTC |
| Phr1.F5-Phr1.R5 | 3,652 | CTTGCCGTGTGTTTGCAATGG | CATCCTTCTCAGTCCATGAGC |
